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Mice were placed into a stereotaxic frame and a craniotomy was performed to allow unilateral microinjection of 5 l of 30 M into the left lateral ventricle (coordinates: +1 mm M/L, 0.5 mm A/P, 2.5 mm D/V from bregma)

As well as, the development of regulatory T cells (Treg) requires exposure to antigen

[2] Tirzepatide dual GIP/GLP-1 agonist pharmacology, PubMed

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